Device

Part:BBa_K189101:Design

Designed by: GuoQiang Chen   Group: iGEM09_Tsinghua   (2009-10-20)


pET linkage group of GenSniper via Bottom Up approach


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 4225
    Illegal XbaI site found at 4592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NheI site found at 4488
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 4658
    Illegal BamHI site found at 4455
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 4225
    Illegal XbaI site found at 4592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 4225
    Illegal XbaI site found at 4592
    Illegal NgoMIV site found at 4690
    Illegal NgoMIV site found at 6278
    Illegal NgoMIV site found at 6438
    Illegal NgoMIV site found at 9485
    Illegal AgeI site found at 437
    Illegal AgeI site found at 1370
    Illegal AgeI site found at 2434
    Illegal AgeI site found at 3314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3011
    Illegal BsaI.rc site found at 4145
    Illegal SapI site found at 7358


Design Notes

None


Source

pET-28a, lambda genome

References